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Liu QN, Ning YS, Zhang YX, Yu N, Zhao CD, Zhan XD, Wu WX, Chen DB, Wei XJ, Wang GL, Cheng SH, Cao LY (2017). OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice. The Plant Cell.

DOI: 10.1105/tpc.16.00650

ABSTRACT

Cullin3-based RING E3 ubiquitin ligases (CRL3), composed of Cullin3 (CUL3), RBX1, and 36 BTB proteins, are involved in plant immunity but the function of CUL3 in the process is 37 largely unknown. Here, we show that rice OsCUL3a is important for the regulation of cell 38 death and immunity. The rice lesion mimic mutant oscul3a displays a significant increase in 39 the accumulation of flg22- and chitin-induced reactive oxygen species, and in 40 pathogenesis-related gene expression as well as resistance to Magnaporthe oryzae and 41 Xanthomonas oryzae pv. oryzae. We cloned the OsCUL3a gene via a map-based strategy and 42 found that the lesion mimic phenotype of oscul3a is associated with the early termination of 43 OsCUL3a protein. Interaction assays showed that OsCUL3a interacts with both OsRBX1a 44 and OsRBX1b to form a multi-subunit CRL in rice. Strikingly, OsCUL3a interacts with and degrades OsNPR1, which acts as a positive regulator of cell death in rice. Accumulation of 46 OsNPR1 protein is greater in the oscul3a mutant than in the wild type. Furthermore, the 47 oscul3a osnpr1 double mutant does not exhibit the lesion mimic phenotype of the oscul3a 48 mutant. Our data demonstrate that OsCUL3a negatively regulates cell death and immunity by 49 degrading OsNPR1 in rice.